Immune Monitoring (Alternative Technologies) (Accredited)


From April 2021, the Immune Monitoring and Immune Monitoring (Alternative Technologies) programmes will merge to form a single Immune Monitoring programme. Participants will not notice any change to the running the programme.


This programme is for Beckman Coulter Aquios flow cytometer users or those using similar technologies. All standard flow cytometer technologies can register via this link for the Immune Monitoring programme. If you are unsure which programme to register, please contact


The enumeration of lymphocyte subsets is important in a variety of conditions such as primary immunodeficiency (eg Severe Combined Immunodeficiency / SCID) or the monitoring of drug therapies such as rituximab in autoimmune disorders. However the most common use is in the monitoring of Human Immunodeficiency Virus / HIV, a secondary immunodeficiency disorder.


To ensure that the programme meets the requirements of all users, the Immune Monitoring (Alternative Technologies) Programme is aimed at those centres using new platform technologies such as the Coulter Aquios system.


The programme issues stabilised whole blood, with laboratories required to determine the lymphocyte subsets (CD3+, CD3+/CD4+, CD3+/CD8+, CD19+ and CD16+/56+) in each sample. Laboratories are requested to report both percentage and absolute values (in cells per microlitre).


Performance is monitored using this data, unless a centre chooses to eschew parameters in which case they would not be performance monitored for those parameters. Two samples are issued per trial and this programme issues trials a minimum of 4 times per annum and a maximum of 6.


Pre issue testing of samples for this programme is subcontracted, although the final decision about sample suitability lies with the EQA provider; no other activities in relation to this EQA programme are subcontracted.



To register for this programme, please click here.

Important Notice for all Immune Monitoring (Alternative Technologies) Participants


From 1st April 2017 (distribution 171801 onwards) the Immune Monitoring (Alternative Technologies) programme will be subject to performance monitoring.


It is essential that your testing repertoire is updated accordingly to reflect current working practices for lymphocyte subsets.


Failure to provide this update could results in classification as a non return if results for these parameters are not submitted

Immune Monitoring (Alternative Technologies) Report Important Announcement – Changes to z score limits

As a result of an internal review we have taken the decision to adjust the performance monitoring limits for quantitative programmes.

• A result between 2.5 and -2.5 will now be classed as satisfactory

• A result between >2.5 and 3.5 or <-2.5 and -3.5 will be classed as an 'Action’ result, which highlights a potential issue to the laboratory. Two ‘Action’ results in a period of 3 samples would result in classification as a ‘Critical’

• A result above 3.5 or below -3.5 is considered to be a ‘critical’ result requiring immediate investigation by the laboratory

Example Immune Monitoring (Alternative Technologies) Report
To view the annotations on this report, please download the PDF and view in a PDF reader such as Adobe Acrobat.
example IM (AT) report.pdf
Adobe Acrobat document [766.5 KB]
Immune Monitoring (Alternative Technologies) Performance Scoring System
IM-AT Performance Scoring System.pdf
Adobe Acrobat document [205.7 KB]
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Last updated 15/12/2021 © UK NEQAS for Leucocyte Immunophenotyping not to be reproduced in all or part without permission.

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