IgH/TCR Clonality Status (Accredited)

 

The diagnosis of lymphoid malignancy can be a challenge that requires many different disciplines of pathology. Molecular analysis of clonality is a valuable supplementary tool, as in principle, all cells of a malignancy have a common clonal origin.

 

In this programme participants are provided with lyophilised cell-lines or patient derived material for IgH and TCR clonality analysis. Participants are asked to submit an overall clonality result, based solely on the IgH or TCR loci, together with details of the methodology. Participants are welcome to submit results of other loci and this information will be included in the send-out report but will not be used for scoring. Instructions for storage, reconstitution and use of the lyophilised samples are included with the samples. Three send-outs are issued per annum, with each send-out consisting of two samples, one for IGH analysis and for TCR, alongside a clinical scenario. Periodically, educational Genemapper traces are also distributed for analysis.

 

Pre issue testing of samples for this programme is subcontracted, although the final decision about sample suitability lies with the EQA provider; no other activities in relation to this EQA programme are subcontracted.

 

To register for this programme, please click here.

Example IgH/TCR Clonality Status Report
IGH TCR 161703 Report Example.pdf
Adobe Acrobat document [1.5 MB]
IgH/TCR Clonality Status Performance Monitoring System
IgH TCR Clonality Performance Criteria.p[...]
Adobe Acrobat document [187.2 KB]
171802 Educational IGH Genemapper Traces
IGH tube A - FR1
IGH tube B - FR2
IGH tube C - FR3
All tubes have been tested in duplicate (rpt)
IGH GeneMapper Traces.pdf
Adobe Acrobat document [281.4 KB]
171802 Educational IGH Polyclonal Controls
IGH Polyclonal Controls.pdf
Adobe Acrobat document [104.6 KB]
171802 Educational IGK Genemapper Traces
IGK tube A VK-JK (KA)
IGK tube B VK/intron -Kde (KB)
All tubes have been tested in duplicate (rpt)
IGK Genemapper Traces.pdf
Adobe Acrobat document [166.2 KB]
171802 Educational IGK Polyclonal Control
IGK Polyclonal Control.pdf
Adobe Acrobat document [109.7 KB]
171802 Educational TCR Genemapper Traces
TCR B tube A VB-JB (BA)
TCR B tube A VB-JB (BB)
TCR B tube C DB-JB (BC)
TCR G tube A V-J (GA)
TCR G tube B V-J (GB)
All tubes have been tested in duplicate (rpt)
TCR Genemapper Traces.pdf
Adobe Acrobat document [310.6 KB]
171802 Educational TCR Polyclonal Control
TCR Polyclonal Control.pdf
Adobe Acrobat document [212.4 KB]
171802 Educational Control Gene DNA Integrity Check
Control Gene DNA Intergrity Check.pdf
Adobe Acrobat document [47.8 KB]
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Last updated 27th September 2017 © UK NEQAS for Leucocyte Immunophenotyping not to be reproduced in all or part without permission.

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