CD34+ Stem Cell Enumeration Programme (Accredited)


In haematopoietic stem cell transplantations the use of CD34+ Stem Cell Enumeration is an essential part of the treatment process, allowing for monitoring of donor mobilisation pre harvest and to ensure sufficient cells are collected to ensure engraftment will occur.


This programme is currently the largest world-wide for CD34+ haematopoietic progenitor cell (hpc) enumeration. The programme uses stabilised peripheral blood obtained from consenting patients following stem cell mobilisation and is suitable for use with whole blood lysis techniques and sequential gating strategies.


Laboratories are requested to report both percentage and absolute values (in cells per microlitre), although performance is only monitored using the absolute values. Two samples are issued per trial and this programme issues trials a minimum of 4 times per annum and a maximum of 6.


Trials for this programme are live/open for a minimum of 3 weeks.

Please Note: Trials issued/closing in August or December are extended by 1 week.

An automated email is sent 2 days prior to the trial closing, to any participant that has not returned results, warning them of the trial closure date. 


No activities in relation to this EQA programme are subcontracted.


To register for this programme, please click here.


CD34+ Stem Cell Enumeration Report Important Announcement – Changes to z score limits

As a result of an internal review we have taken the decision to adjust the performance monitoring limits for quantitative programmes.

• A result between 2.5 and -2.5 will now be classed as satisfactory

• A result between >2.5 and 3.5 or <-2.5 and -3.5 will be classed as an 'Action’ result, which highlights a potential issue to the laboratory. Two ‘Action’ results in a period of 3 samples would result in classification as a ‘Critical’

• A result above 3.5 or below -3.5 is considered to be a ‘critical’ result requiring immediate investigation by the laboratory

Example CD34+ Stem Cell Enumeration Report
To view the annotations on this report, please download the PDF and view in a PDF reader such as Adobe Acrobat.
CD34 Stem cell enumeration annotated rep[...]
Adobe Acrobat document [828.9 KB]
CD34+ Stem Cell Enumeration Performance Scoring System
CD34 Performance Monitoring System.pdf
Adobe Acrobat document [139.7 KB]
CD34 242502 Coversheet
CD34 242502.pdf
Adobe Acrobat document [161.0 KB]
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Last updated 14/06/2024 © UK NEQAS for Leucocyte Immunophenotyping not to be reproduced in all or part without permission.

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